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Biology
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单细胞转染鸡胚
JoVE Journal
Biology
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JoVE Journal
Biology
Single Cell Transfection in Chick Embryos
Please note that all translations are automatically generated.
Click here for the English version.
单细胞转染鸡胚
DOI:
10.3791/2133-v
•
08:46 min
•
September 25, 2010
•
Raz Ben-Yair
,
Chaya Kalcheim
1
Department of Medical Neurobiology
,
Hadassah Medical School – Hebrew University
Chapters
00:00
Title
00:57
Introduction
01:21
Micropipette, DNA, and Embryo Preparation
04:15
Loading Micropipettes and Microinjection
06:49
Representative Injection Results
08:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
使用精尖微量注入鸡体节或神经管的子域的质粒DNA。质粒的浓度调整,以产生单一的转染细胞。然后,我们允许的细胞发育成无性系种群。
Tags
Single Cell Transfection
Chick Embryos
Developmental Biology
Lineage Diversification
Molecular Mechanisms
Transfection Methods
Single Progenitors
In-ovo Transfection
Lineage Tracing
Genetic Manipulation
Somite
Neural Tube
Epithelium
Sporadic Transfection
Clonal Populations
Reporters
Genetic Manipulation Tools
Electroporation Method
In-vivo Models
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