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Visualizzazione dei nervi larvali segmentale in 3 Rd Instar Drosophila I preparativi larvali
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
DOI:

07:18 min

September 29, 2010

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Chapters

  • 00:00Title
  • 00:49Introduction
  • 01:10Dissection of 3rd Instar Larvae
  • 03:20Fixation and Antibody Staining of the Dissected Larva
  • 05:06Mounting and Visualization of the Dissected Larva
  • 06:17Representative Images of Drosophila Larvae Segmental Nerves
  • 06:49Conclusion

Summary

Automatic Translation

Larve di Drosophila melanogaster fornire un sistema modello ideale per studiare i meccanismi di trasporto assonale in nervi larvale segmentale. Utilizzando questa procedura, 3 ° larve instar portatori di mutazioni varie può essere paragonato a larve wild-type.

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