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Visualisierung von Larven segmentalen Nerven in 3 Rd Instar Drosophila Larven Vorbereitungen
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
DOI:

07:18 min

September 29, 2010

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Chapters

  • 00:00Title
  • 00:49Introduction
  • 01:10Dissection of 3rd Instar Larvae
  • 03:20Fixation and Antibody Staining of the Dissected Larva
  • 05:06Mounting and Visualization of the Dissected Larva
  • 06:17Representative Images of Drosophila Larvae Segmental Nerves
  • 06:49Conclusion

Summary

Automatic Translation

Drosophila melanogaster-Larven bieten ein ideales Modellsystem, um die Mechanismen der axonalen Transport innerhalb Larven segmentalen Nerven zu untersuchen. Mit diesem Verfahren können 3 rd Larvenstadium, die verschiedene Mutationen zu Wildtyp-Larven verglichen werden.

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