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Chapters
- 00:00Estimation of bead concentrations
- 00:10Introduction
- 00:46Reagents
- 01:27Coating beads with HUVEC cells
- 03:15Washing beads
- 05:34Estimation of bead concentrations
- 08:02Forming the fibrin matrix
- 10:22Layering lung fibroblasts atop the fibrin gel
Cette vidéo montre le protocole d'un essai angiogenèse in vitro que les stades récapitule plusieurs de l'angiogenèse. Time-lapse images de la germination, la formation de lumière, la ramification et anastomose - caractéristiques essentielles de l'angiogenèse - sont présentés.
Tags
Optimized Fibrin Gel Bead AssayStudy Of AngiogenesisMulti-step ProcessAngiogenic StimuliNew VesselsExisting VasculatureDegradation Of Basement MembraneProliferation And Migration Of Endothelial Cells (EC)Extracellular MatrixAlignment Of EC Into CordsBranchingLumen FormationAnastomosisFormation Of New Basement MembraneIn Vitro AssaysMimic Certain Stages Of AngiogenesisVessels In VivoHuman Umbilical Vein ECFibroblastsIn Vitro Angiogenesis AssayCytodex MicrocarriersFibrin GelSoluble FactorsEC SproutingPhase-contrast MicroscopyTime-lapse Microscopy
