JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
优化的纤维蛋白凝胶珠检测血管生成的研究
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis
Please note that all translations are automatically generated.
Click here for the English version.
优化的纤维蛋白凝胶珠检测血管生成的研究
DOI:
10.3791/186-v
•
14:14 min
•
April 29, 2007
•
Martin N. Nakatsu
,
Jaeger Davis
,
Christopher C.W. Hughes
1
Department of Molecular Biology and Biochemistry
,
University of California, Irvine (UCI)
Chapters
00:00
Estimation of bead concentrations
00:10
Introduction
00:46
Reagents
01:27
Coating beads with HUVEC cells
03:15
Washing beads
05:34
Estimation of bead concentrations
08:02
Forming the fibrin matrix
10:22
Layering lung fibroblasts atop the fibrin gel
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本视频演示了一个协议,在体外血管生成试验,概括的血管生成的几个阶段。显示图像时间的推移,发芽,管腔形成,分支和吻合 - 血管生成的关键特征 - 。
Tags
Optimized Fibrin Gel Bead Assay
Study Of Angiogenesis
Multi-step Process
Angiogenic Stimuli
New Vessels
Existing Vasculature
Degradation Of Basement Membrane
Proliferation And Migration Of Endothelial Cells (EC)
Extracellular Matrix
Alignment Of EC Into Cords
Branching
Lumen Formation
Anastomosis
Formation Of New Basement Membrane
In Vitro Assays
Mimic Certain Stages Of Angiogenesis
Vessels In Vivo
Human Umbilical Vein EC
Fibroblasts
In Vitro Angiogenesis Assay
Cytodex Microcarriers
Fibrin Gel
Soluble Factors
EC Sprouting
Phase-contrast Microscopy
Time-lapse Microscopy
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
测试的速度和运动方向的视觉灵敏度在蜥蜴
克里斯托弗休斯:在体外血管生成的研究模型(面试)
计算机生成的动物模型的刺激
分离纯化
果蝇
外周神经元
大鼠肠系膜血管生成法
做自己动手恢复冻存样品的设备不慎掉落到低温储罐
琼脂糖凝胶电泳分离DNA片段
内皮细胞小管形成实验的<em>在体外</em>研究血管生成
凝胶电泳迁移分析(EMSA)为RNA,蛋白质相互作用的研究:IRE / IRP示例
一个简单的生物测定血管内皮生长因子的评价
Read Article