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Chapters
- 00:00Title
- 00:54Introduction
- 02:09Microdissection of Explants
- 07:16Microdissection and Culture of Central Explants
- 09:18Harvesting the Explants for Protein Analysis
- 10:19Harvesting the Explants for Immunofluorescence
- 12:15Immunofluorescence and Protein Analysis Results
- 15:54Conclusion
Explantes da região central da lente de ratos epitélio diferenciar de forma síncrona quando cultivadas na presença de FGF-2. Microscopia de imunofluorescência de tais culturas pode fornece informações romance sobre a expressão gênica e eventos de sinalização associado com a diferenciação terminal.
Tags
PreparationCultureRat Lens Epithelial ExplantsTerminal DifferentiationAnterior SurfaceOcular LensEpithelial CellsProliferationAnnular ZoneCiliary BodyMigrationPosterior ArrayLens Fiber CellsBulk Of The LensIn Vitro InductionFGF-2Neonatal RatsLens CapsuleDissecting TweezersPeripheral RegionsCentral AreaFGF-2 ConcentrationTime CourseSignalingGene ExpressionMolecular TechniquesBiochemical TechniquesPharmacological TechniquesImmunofluorescence Microscopy
