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大鼠晶状体上皮细胞外植体的制备及文化学习终末分化
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation
Please note that all translations are automatically generated.
Click here for the English version.
大鼠晶状体上皮细胞外植体的制备及文化学习终末分化
DOI:
10.3791/1519-v
•
15:02 min
•
September 22, 2009
•
Peggy S. Zelenka
,
Chun Y. Gao
,
Senthil S. Saravanamuthu
1
Laboratory of Molecular and Developmental Biology
,
National Eye Institute (NEI), National Institutes of Health (NIH)
Chapters
00:00
Title
00:54
Introduction
02:09
Microdissection of Explants
07:16
Microdissection and Culture of Central Explants
09:18
Harvesting the Explants for Protein Analysis
10:19
Harvesting the Explants for Immunofluorescence
12:15
Immunofluorescence and Protein Analysis Results
15:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在FGF - 2的存在培养的大鼠晶状体上皮细胞的中部地区的外植体分化同步。这种文化免疫荧光显微镜,可以提供有关新的信息与终末分化相关的基因表达和信号事件。
Tags
Preparation
Culture
Rat Lens Epithelial Explants
Terminal Differentiation
Anterior Surface
Ocular Lens
Epithelial Cells
Proliferation
Annular Zone
Ciliary Body
Migration
Posterior Array
Lens Fiber Cells
Bulk Of The Lens
In Vitro Induction
FGF-2
Neonatal Rats
Lens Capsule
Dissecting Tweezers
Peripheral Regions
Central Area
FGF-2 Concentration
Time Course
Signaling
Gene Expression
Molecular Techniques
Biochemical Techniques
Pharmacological Techniques
Immunofluorescence Microscopy
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