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Journal
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Immunology and Infection
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補体の枯渇により特定の細胞集団の枯渇
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Depletion of Specific Cell Populations by Complement Depletion
Please note that all translations are automatically generated.
Click here for the English version.
補体の枯渇により特定の細胞集団の枯渇
DOI:
10.3791/1487-v
•
06:17 min
•
February 05, 2010
•
Bonnie N. Dittel
1
BloodCenter of Wisconsin
,
Blood Research Institute
Chapters
00:00
Title
00:45
Introduction
01:18
Preparation of Rabbit Complement and Antibody Selection
02:22
Complement Depletion
05:06
Results of the Complement Depletion
05:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
効果的に浄化がしばしば必要とされる免疫細胞集団の機能を研究する。補体の枯渇は、高純度と免疫細胞集団を単離するための高速かつ安価な手法です。
Tags
Depletion
Specific Cell Populations
Complement Depletion
Immune Cell Purification
Real-time PCR
Microarray Analysis
Fluorescent Activated Cell Sorting (FACS)
Magnetic Bead Separation
Complement System
Plasma Proteins
Proteolytic Cascade
Membrane-attack Complex
Cell Death
Classical Pathway
IgM Antibodies
IgG Antibodies
Monoclonal Antibodies (mAb)
Antigen-specific Manner
Rabbit Complement
Incubation
Washing
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