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Journal
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Immunology and Infection
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补消耗的特定细胞群的枯竭
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Depletion of Specific Cell Populations by Complement Depletion
Please note that all translations are automatically generated.
Click here for the English version.
补消耗的特定细胞群的枯竭
DOI:
10.3791/1487-v
•
06:17 min
•
February 05, 2010
•
Bonnie N. Dittel
1
BloodCenter of Wisconsin
,
Blood Research Institute
Chapters
00:00
Title
00:45
Introduction
01:18
Preparation of Rabbit Complement and Antibody Selection
02:22
Complement Depletion
05:06
Results of the Complement Depletion
05:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
为了有效地研究其净化往往需要的人群的免疫细胞的功能。补枯竭是一个快速和廉价的技术,高纯度的免疫细胞群的隔离。
Tags
Depletion
Specific Cell Populations
Complement Depletion
Immune Cell Purification
Real-time PCR
Microarray Analysis
Fluorescent Activated Cell Sorting (FACS)
Magnetic Bead Separation
Complement System
Plasma Proteins
Proteolytic Cascade
Membrane-attack Complex
Cell Death
Classical Pathway
IgM Antibodies
IgG Antibodies
Monoclonal Antibodies (mAb)
Antigen-specific Manner
Rabbit Complement
Incubation
Washing
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