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ニューロンとグリアの開発研究ラットの脳の集計文化の調製
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Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies
Please note that all translations are automatically generated.
Click here for the English version.
ニューロンとグリアの開発研究ラットの脳の集計文化の調製
DOI:
10.3791/1304-v
•
12:03 min
•
September 30, 2009
•
Hisami Koito
,
Jianrong Li
1
Department of Veterinary Integrative Biosciences
,
Texas A&M University (TAMU)
Chapters
00:00
Title
00:52
Introduction
01:27
Preparing for Dissection
02:02
Harvesting the E16 Rat Embryos
03:19
Isolating the Forebrains
04:25
Digesting and Triturating the Forebrain Tissue
06:09
Washing, Filtering, and Counting the Dissociated Cells
07:03
Culturing the Dissociated Cells to Promote Aggregation
07:54
Preparing the Coverslips and Washing the Aggregates
09:15
Seeding the Coverslips with the Aggregates
11:06
Representative Connections Formed Among Aggregates
11:29
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
胎児ラット脳の集合体の培養系のためのプロトコルが記述されています。凝集体の多能性前駆細胞は、開発し、神経細胞、アストロサイト、オリゴデンドロサイトに分化することができる。
Tags
Rat Brain Aggregate Cultures
Neuron Development Studies
Glia Development Studies
In Vitro System
Central Nervous System (CNS)
Progenitors
Mature Neurons
Glia
Axo-glial Interactions
Multipotent Progenitors
Oligodendroglial Lineage Cells
Cellular And Molecular Level
Serum-free Medium
Embryonic Rat Forebrains
Neuron-glia Interaction
CNS Myelination
Isolate And Grow CNS Aggregate Cultures
E16 Rat Brain
Regular Dissociated Neuronal Cultures
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