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制备大鼠脑组织神经元和神经胶质发展研究总结文化
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Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies
Please note that all translations are automatically generated.
Click here for the English version.
制备大鼠脑组织神经元和神经胶质发展研究总结文化
DOI:
10.3791/1304-v
•
12:03 min
•
September 30, 2009
•
Hisami Koito
,
Jianrong Li
1
Department of Veterinary Integrative Biosciences
,
Texas A&M University (TAMU)
Chapters
00:00
Title
00:52
Introduction
01:27
Preparing for Dissection
02:02
Harvesting the E16 Rat Embryos
03:19
Isolating the Forebrains
04:25
Digesting and Triturating the Forebrain Tissue
06:09
Washing, Filtering, and Counting the Dissociated Cells
07:03
Culturing the Dissociated Cells to Promote Aggregation
07:54
Preparing the Coverslips and Washing the Aggregates
09:15
Seeding the Coverslips with the Aggregates
11:06
Representative Connections Formed Among Aggregates
11:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
胚胎大鼠脑组织总文化系统的一个协议描述。在聚集的多能祖细胞可以发展和分化成神经元,星形胶质细胞和少突胶质细胞。
Tags
Rat Brain Aggregate Cultures
Neuron Development Studies
Glia Development Studies
In Vitro System
Central Nervous System (CNS)
Progenitors
Mature Neurons
Glia
Axo-glial Interactions
Multipotent Progenitors
Oligodendroglial Lineage Cells
Cellular And Molecular Level
Serum-free Medium
Embryonic Rat Forebrains
Neuron-glia Interaction
CNS Myelination
Isolate And Grow CNS Aggregate Cultures
E16 Rat Brain
Regular Dissociated Neuronal Cultures
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