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Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells
October 27, 2023
使用荧
光成像快速体外细胞毒性评估表达嵌合抗原受体的 Jurkat
01:44 min
•
接种 CFSE 标记的肿瘤细胞并共培养表达 CAR 的 Jurkats 进行体外细胞毒性评估
03:39 min
•
制备用于 CAR-Jurkat 共培养与 CFSE 标记的肿瘤细胞的荧光成像和细胞活力评估的板
04:00 min
•
Siddharth Subham*
1,2,3
,
John D. Jeppson*
1
,
Benjamin K. Gibbs
,
Jacqueline Babai
,
Riza Alker
,
Andrew K. Godwin
5,6
,
David Akhavan
2,3
1
Department of Radiation Oncology
,
University of Kansas Cancer Center
,
2
Department of Cancer Biology
,
University of Kansas Cancer Center
,
3
BioEngineering Program
,
University of Kansas
,
4
Department of Pathology and Laboratory Medicine
,
University of Kansas Medical Center
,
5
University of Kansas Cancer Center
,
6
Kansas Institute for Precision Medicine
,
University of Kansas Medical Center
Summary
Automatic Translation
English (Original)
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一种评估靶向单个肿瘤抗原的表达嵌合抗原受体 (CAR) 的 Jurkat 细胞定量肿瘤细胞杀伤的方案。该方案可用作筛选平台,用于在外周血来源的T细胞中确认之前快速优化CAR铰链结构。
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