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Journal
/
Cancer Research
/
Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells
/
Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation
Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation
Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells
DOI:
10.3791/200792-v
•
03:39 min
•
October 27, 2023
•
Siddharth Subham*
1,2,3
,
John D. Jeppson*
1
,
Benjamin K. Gibbs
,
Jacqueline Babai
,
Riza Alker
,
Andrew K. Godwin
5,6
,
David Akhavan
2,3
1
Department of Radiation Oncology
,
University of Kansas Cancer Center
,
2
Department of Cancer Biology
,
University of Kansas Cancer Center
,
3
BioEngineering Program
,
University of Kansas
,
4
Department of Pathology and Laboratory Medicine
,
University of Kansas Medical Center
,
5
University of Kansas Cancer Center
,
6
Kansas Institute for Precision Medicine
,
University of Kansas Medical Center
Tags
CFSE-labeling
Tumor Cells
CAR-expressing Jurkats
In Vitro Cytotoxicity
Cell Culture
Trypsinization
Cell Counting
Cell Transfer
Plate Preparation
Effector-to-target Ratio
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