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Sigma的非特异性蛋白酶活性测定 - 酪蛋白作为基质
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Sigma’s Non-specific Protease Activity Assay – Casein as a Substrate
Please note that all translations are automatically generated.
Click here for the English version.
Sigma的非特异性蛋白酶活性测定 - 酪蛋白作为基质
DOI:
10.3791/899-v
•
11:37 min
•
September 17, 2008
•
Carrie Cupp-Enyard
1
Sigma Aldrich
Chapters
00:00
Title
01:23
Introduction
01:52
Preparation of Reagents
04:33
Setting Up the Protease Assay and Standard Curves
08:12
Measuring Absorbance and Calculating Enzyme Activity
10:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
蛋白酶打破肽键。在实验室中,它往往是必要的,来衡量和/或比较蛋白酶的活性。 Sigma的非特异性的蛋白酶活性检测可作为一个标准化的程序,以确定蛋白酶的活性。
Tags
Sigma
Non-specific Protease Activity Assay
Casein Substrate
Quality Control Procedures
Amino Acid Tyrosine
Peptide Fragments
Folin And Ciocalteus Phenol
Folin’s Reagent
Blue Colored Chromophore
Absorbance Value
Spectrophotometer
Tyrosine Release
Protease Activity
Standard Curve
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Micromoles
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