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全基因组阵列比较基因组杂交技术示范

JoVE Journal
Biology

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JoVE Journal Biology

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

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全基因组阵列比较基因组杂交技术示范

DOI:

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16:37 min

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August 05, 2008

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Jennifer Y. Kennett, Spencer K. Watson, Heather Saprunoff, Cameron Heryet, Wan L. Lam

¹Department of Cancer Genetics,BC Cancer Research Centre, ²Deeley Research Centre,BC Cancer Agency, ³Photography/Video Production, Multi-Media Services,BC Cancer Agency

Chapters

00:04Title
01:33Day 1: DNA Quality
03:21Probe Labeling
06:43Day 2: Sample Clean-Up
09:29Calculation of Incorporations
10:55Array Hybridization
12:44Array Washing
15:34Array Scanning

Summary

Automatic Translation

这个视频是一个全基因组平铺路径阵列比较基因组杂交，扫描整个人类基因组只用25-100纳克的DNA，可以从多种来源，包括档案福尔马林固定材料隔离技术示范的杂交协议。

Tags

Array CGH Comparative Genomic Hybridization Genetic Alterations Cancer Genetic Diseases SMRT Array BAC Clones DNA Segments Gene Dosage Competitive Hybridization Fluorescent Dyes SeeGH Software Log2 Signal Intensity Ratio Chromosomal Position DNA Rearrangement Events

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