JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
人类胚胎干细胞:启动文化冷冻细胞
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Human ES cells: Starting Culture from Frozen Cells
Please note that all translations are automatically generated.
Click here for the English version.
人类胚胎干细胞:启动文化冷冻细胞
DOI:
10.3791/86-v
•
02:47 min
•
November 09, 2006
•
Erin Trish
,
John Dimos
,
Kevin Eggan
1
Department of Molecular and Cell Biology
,
Harvard
Chapters
00:03
Prepare media
00:17
Take cells from freezer and thaw
01:58
Start culture
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们证明,我们的实验室如何从冻结的股票开始一个色调的人类胚胎干细胞线文化。
Tags
Human ES Cells
Culture
Frozen Cells
Embryonic Stem Cell Line
HuES Media
Mouse Embryonic Fibroblast Feeder Cells
Serum
Cell Debris
Liquid Nitrogen Storage
-80C Freezer
Thawing
Freezing Media
DMSO
Differentiation
Suspension
Seeding
MEF Plate
Incubator
Media Replacement
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
冻结人类胚胎干细胞
人类胚胎干细胞(ES细胞)的繁殖
从人类胚胎干细胞的染色质免疫沉淀
文化和维护人类胚胎干细胞
成人从人体皮肤的上皮干细胞的分离和培养
前
初级人类输卵管上皮细胞
的体外
培养
诱导上皮和分析间质转化
从成年小鼠切牙牙源性上皮干细胞的分离和培养
从人类体细胞与仙台病毒高效的新一代人类诱导多能干细胞
人外周血代文化血液的产物内皮细胞
Read Article