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半变性洗涤剂琼脂糖凝胶电泳筛选淀粉样蛋白聚合
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Biology
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JoVE Journal Biology
Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
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半变性洗涤剂琼脂糖凝胶电泳筛选淀粉样蛋白聚合

DOI:
10.3791/838-v

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10:03 min

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July 16, 2008

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,
1Whitehead Institute for Biomedical Research, 2Department of Biology,MIT – Massachusetts Institute of Technology, 3Howard Hughes Medical Institute

Chapters

  • 00:00Title
  • 00:48Introduction
  • 01:41Preparing the Gel
  • 03:10Preparing The Samples
  • 06:23Capillary Gel Transfer
  • 09:15Typical Blotting Results
  • 09:58Conclusion

Summary

Automatic Translation

Automatic Translation

sdd的年龄是一个有用的技术检测和表征状聚合物在细胞中的淀粉样蛋白。在这里，我们展示了一个适应，使这种技术适合大规模应用。

Tags

Amyloid AggregationProtein MisfoldingPrionsConformationally Self-templating ProteinsLower OrganismsInsolubilityStructural HeterogeneitySemi-Denaturing Detergent-Agarose Gel Electrophoresis (SDD-AGE)Size-based ResolutionDetergent InsolubilityAmyloid PolymersDetectionCharacterizationConformational VariantsLarge-scale ApplicationsScreens For Prions And Amyloidogenic ProteinsSDD-AGE MethodCapillary TransferReliabilityEase Of UseSDS-insoluble Conformers

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