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专访:HIV - 1前病毒DNA切除使用一个演进的重组酶
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JoVE Journal
Biology
Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
Please note that all translations are automatically generated.
Click here for the English version.
专访:HIV - 1前病毒DNA切除使用一个演进的重组酶
DOI:
10.3791/793-v
•
10:20 min
•
June 16, 2008
•
Joachim Hauber
1
Department of Cell Biology and Virology
,
Heinrich-Pette-Institute for Experimental Virology and Immunology, University of Hamburg
Chapters
00:07
Title
00:59
What are limitations of today’s antiretroviral therapies
01:41
Excision of proviral DNA. Targeting LTR specific sequences.
03:39
Addressing the problem of multiple HIV isolates.
04:35
Generation of site-specific Tre recombinase via directed molecular evolution.
05:49
Delivery of Tre recombinase, HIV-1 proviral DNA excision, and elimination of HIV-1 viruses.
06:39
Open questions.
08:21
Towards a clinical application.
Summary
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Automatic Translation
现有的HIV - 1战略采取行动来抑制病毒的生命周期,但不能有效地消除感染。在这里,我们证明了一个精心设计的重组可以有效地消费综合HIV - 1前病毒从感染细胞的基因组DNA。
Tags
HIV-1
Proviral DNA Excision
Evolved Recombinase
Treatment Strategies
Resistant Strains
Tre Recombinase
LoxLTR
Viral Pseudotypes
HeLa Cells
Virus Production
Proof Of Principle
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