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在体外和体内生物发光记者人类胚胎干细胞的基因显像
JoVE Journal
Biology
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JoVE Journal Biology
In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
DOI:

12:19 min

May 02, 2008

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Chapters

  • 00:00Imaging Teratoma Formation
  • 01:07Introduction
  • 01:47Getting Started: Review of Stem Cell Culture and Imaging Set-Up
  • 03:33In Vitro Bioluminescence Imaging of Human Embryonic Stem Cells
  • 07:47In vivo Bioluminescence Imaging of Injected hESC’s
  • 11:17Conclusion

Summary

Automatic Translation

分子成像技术在干细胞疗法的兴趣与日俱增,是理想的监测干细胞移植后的行为。荧光素酶基因已启用非侵入性,重复评估细胞的存活,位置,并在体内扩散。本视频将演示如何跟踪生活在一个小鼠胚胎干细胞增殖。

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