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一个人脑组织中的染色质含量
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A Chromatin Assay for Human Brain Tissue
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一个人脑组织中的染色质含量
DOI:
10.3791/717-v
•
11:31 min
•
March 21, 2008
•
Anouch Matevossian
,
Schahram Akbarian
1
Psychiatry, Brudnick Neuropsychiatric Research Institute
,
University of Massachusetts Medical School
Chapters
00:00
Title
00:14
Introduction
01:11
Micrococcal Nuclease Treatment
02:27
Hypotonisation
07:09
Elution
08:00
Protein Digestion and Pheno Chloroform Extraction
10:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
直到最近,表达对人类大脑的研究仅限于RNA或蛋白质的定量。本文中所描述的染色质免疫沉淀技术,将有可能映射在死后大脑基因表达的组蛋白甲基化和其他后生监管。
Tags
Chromatin Assay
Neuropsychiatric Illnesses
Schizophrenia
Bipolar Disease
Autism
Epigenetic Alterations
Gene Expression
Molecular Pathology
Postmortem Brain Research
MRNA Quantification
Protein Quantification
Autolysis Time
Chromatin Structures
Nucleosomal Organization
DNA:core Histone Binding
Methylation Pattern
Covalent Modifications
Core Histones
Genomic Loci
Native Chromatin Immunoprecipitation (NChIP)
Frozen Brain Specimens
Micrococcal Nuclease Digestion
QPCR
Epigenetic Mechanisms
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