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Biology
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肌动蛋白共沉淀测定; F -肌动蛋白结合蛋白分析
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JoVE Journal
Biology
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
Please note that all translations are automatically generated.
Click here for the English version.
肌动蛋白共沉淀测定; F -肌动蛋白结合蛋白分析
DOI:
10.3791/690-v
•
07:53 min
•
March 28, 2008
•
Jyoti Srivastava
,
Diane Barber
1
Department of Cell and Tissue Biology
,
University of California, San Francisco – UCSF
Chapters
00:00
Title
00:42
Introduction
01:44
Preparation of Actin Filaments
02:49
Preparation of Protein and Binding of Actin
04:26
Running the Gel
06:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
蛋白质结合丝状肌动蛋白通过不同的肌动蛋白结合模块(F - actin的)。在这个视频中,我们共同沉淀,这是一个经常使用的分析,结合F -肌动蛋白的蛋白质或特定域在体外实验证明肌动蛋白的过程。
Tags
Actin Co-sedimentation Assay
Protein Binding
F-actin
Actin Cytoskeleton
Cellular Processes
Dynamic Structure
Remodeling
Protein Analysis
In Vitro Assay
Ultracentrifugation
Protein Co-sedimenting
Actin Binding Affinities
Competition Assays
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