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与非侵入性检测荧光染料标记的干细胞与光学成像
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JoVE Journal
Biology
Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
Please note that all translations are automatically generated.
Click here for the English version.
与非侵入性检测荧光染料标记的干细胞与光学成像
DOI:
10.3791/686-v
•
07:42 min
•
April 02, 2008
•
Sophie Boddington
,
Tobias D. Henning
,
Elizabeth J. Sutton
,
Heike E. Daldrup-Link
1
Contrast Agent Research Group at the Center for Molecular and Functional Imaging, Department of Radiology, University of California San Francisco
Chapters
00:01
Title
00:55
Introduction
01:41
Labeling of Human Mesenchymal Stem Cells with Fluorescent Dye DiD
03:12
Labeling of Human Embryonic Stem Cells with the Fluorescent Dye ICG
06:23
Imaging Stem Cells in vivo
06:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该视频显示技术,人类胚胎干细胞和骨髓基质干细胞与荧光染料标签。这种技术可以用于移植的干细胞在体内追踪与光学成像和荧光显微镜观察组织病理学的相关性。
Tags
Labeling
Stem Cells
Fluorescent Dyes
Non-invasive Detection
Optical Imaging
In Vivo Monitoring
Ex Vivo Labeling
Intravenous Injection
Visualization
Target Organs
Pathologies
Human Mesenchymal Stem Cells (hMSC)
Lipophilic Fluorescent Dye DiD
Human Embryonic Stem Cells (hESC)
FDA Approved Fluorescent Dye Indocyanine Green (ICG)
Uptake Mechanism
Adherence
Diffusion
Phospholipid Cell Membrane Bilayer
Labeling Efficiency
Serum-free Media
Serum-containing Media
Transfection Agent Protamine Sulfate
Contrast Agent Uptake
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