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Single Cell Nuclei Isolation from IMAT for Studying Gene Expression Profiles

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Author Spotlight: Deciphering the Cellular Mysteries of Intermuscular Adipose Tissue in Humans

JoVE Journal
Biology

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JoVE Journal Biology

Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing

Author Spotlight: Deciphering the Cellular Mysteries of Intermuscular Adipose Tissue in Humans

Single Cell Nuclei Isolation from IMAT for Studying Gene Expression Profiles

DOI:

10.3791/66784-v

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02:07 min

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May 03, 2024

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Line O. Elingaard-Larsen², Katie L. Whytock, Adeline Divoux, Meghan Hopf, Erin E. Kershaw, Jamie N. Justice, Bret H. Goodpaster, Nancy E. Lane, Lauren M. Sparks

¹Translational Research Institute,AdventHealth, ²Steno Diabetes Center Copenhagen, ³Division of Endocrinology and Metabolism,University of Pittsburgh, ⁴Gerontology and Geriatric Medicine,Wake Forest University School of Medicine, ⁵Department of Internal Medicine – Rheumatology, Allergy, and Clinical Immunology,University of California Davis Health

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Single-nuclei RNA Sequencing Adipocyte Skeletal Muscle Lipid-laden Nuclei Isolation Droplet-based Sequencing Cell Type-specific Analysis

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