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从海兔的神经细胞培养的高分辨率成像生长锥
JoVE Journal
Biology
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JoVE Journal Biology
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones
DOI:

16:29 min

February 20, 2008

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Chapters

  • 00:00Title
  • 00:35Introduction
  • 02:13Sacrifice, Dissection, and Digestion of Aplysia Abdominal Ganglion
  • 04:37Dissection of Aplysia Bag Cells from Abdominal Ganglion and Plating
  • 10:17Setting up Aplysia bag cells for live imaging
  • 12:35Visualization of Growth Cone Morphology, Motility, and Cytoskeletal
  • 15:48Conclusion

Summary

Automatic Translation

海兔californica神经元发展大文化的生长锥,生长锥的运动和指导的高分辨率成像的优秀。在这里,我们提出了一种海兔袋细胞神经元的剥离和电镀以及设立一个活细胞成像室的协议。

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