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从海兔的神经细胞培养的高分辨率成像生长锥
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Biology
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones
Please note that all translations are automatically generated.
Click here for the English version.
从海兔的神经细胞培养的高分辨率成像生长锥
DOI:
10.3791/662-v
•
16:29 min
•
February 20, 2008
•
Aih Cheun Lee
,
Boris Decourt
,
Daniel M. Suter
1
Department of Biological Sciences
,
Purdue University
Chapters
00:00
Title
00:35
Introduction
02:13
Sacrifice, Dissection, and Digestion of Aplysia Abdominal Ganglion
04:37
Dissection of Aplysia Bag Cells from Abdominal Ganglion and Plating
10:17
Setting up Aplysia bag cells for live imaging
12:35
Visualization of Growth Cone Morphology, Motility, and Cytoskeletal
15:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
海兔californica神经元发展大文化的生长锥,生长锥的运动和指导的高分辨率成像的优秀。在这里,我们提出了一种海兔袋细胞神经元的剥离和电镀以及设立一个活细胞成像室的协议。
Tags
Neuronal Cell Cultures
Aplysia
High-resolution Imaging
Growth Cones
Guidance Cues
Directional Movement
Live Cell Imaging
Protein Dynamics
Temporal Resolution
Spatial Resolution
F-actin Dynamics
Microtubule Dynamics
Sea Hare Neurons
Micromanipulation
Biophysical Measurements
Cytoplasmic Regions
Cytoskeletal System
Microinjection
Growth Cone Motility
Imaging Chamber
Article
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