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Journal
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Cancer Research
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患者来源乳腺类器官的建立和培养
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Establishment and Culture of Patient-Derived Breast Organoids
Please note that all translations are automatically generated.
Click here for the English version.
患者来源乳腺类器官的建立和培养
DOI:
10.3791/64889-v
•
08:29 min
•
February 17, 2023
•
Disha Aggarwal
2
,
Suzanne Russo
,
Payal Naik
,
Sonam Bhatia
,
David L. Spector
2
1
Cold Spring Harbor Laboratory
,
Cold Spring Harbor
,
2
Genetics Graduate Program
,
Stony Brook University
Chapters
00:04
Introduction
01:12
Establishing Breast Tumor/Normal Organoids from Resected Tissue
03:59
Passaging and Expanding Patient-Derived Breast Organoids in Culture
06:39
Results: Morphology and Growth Rate of Patient-Derived Breast Tumor Organoid Lines
07:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里提供了一个详细的方案,用于从患者来源的乳腺肿瘤切除术或正常乳腺组织中建立人乳腺类器官。该协议为培养、冷冻和解冻人类患者来源的乳腺类器官提供了全面的分步说明。
Tags
Patient-derived Organoids
Breast Tumor Organoids
3D In Vitro Model
Cellular Heterogeneity
Collagenase IV
Red Blood Cell Lysis
Basement Membrane Matrix
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