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高级别浆液性卵巢癌患者来源类器官的生成和培养
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Cancer Research
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JoVE Journal Cancer Research
Generation and Culturing of High-Grade Serous Ovarian Cancer Patient-Derived Organoids
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高级别浆液性卵巢癌患者来源类器官的生成和培养

DOI:
10.3791/64878-v

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07:19 min

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January 06, 2023

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, , , , , , ,
1Washington University in St. Louis, 2University of California San Francisco

Chapters

  • 00:05Introduction
  • 00:51Harvesting Organoids from Tissue
  • 03:58Passaging of Organoids
  • 05:05Freezing and Thawing of Organoids
  • 06:22Results: Generating Patient-Derived Ovarian Cancer Organoids from Patient Tumors, Ascites, and Pleural Fluid Samples
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

患者来源类器官（PDO）是一种三维（3D）培养物，可以在 体外模拟肿瘤环境。在高级别浆液性卵巢癌中，PDO代表了研究新型生物标志物和治疗方法的模型。

Tags

Ovarian CancerPatient-derived OrganoidsTumor MicroenvironmentCell-cell InteractionsNovel TherapeuticsPredictive BiomarkersTumor HeterogeneityCollagenaseDNaseCell FiltrationCell PelletRBC Lysis

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