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Journal
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Immunology and Infection
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使用牛单核细胞来源的树突状细胞测定疫苗免疫原性
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JoVE Journal
Immunology and Infection
Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
Please note that all translations are automatically generated.
Click here for the English version.
使用牛单核细胞来源的树突状细胞测定疫苗免疫原性
DOI:
10.3791/64874-v
•
12:54 min
•
May 19, 2023
•
Fatima Liaqat
,
Richard Thiga Kangethe
,
Rudolf Pichler
,
Bo Liu
,
Johann Huber
,
Viskam Wijewardana
,
Giovanni Cattoli
,
Luca Porfiri
1
Animal Production and Health Section, Joint Food and Agriculture Organization (FAO)/International Atomic Energy Agency (IAEA) Centre of Nuclear Techniques in Food and Agriculture
,
International Atomic Energy Agency
,
2
Teaching and Research Farm Kremesberg, Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health
,
University of Veterinary Medicine, Vienna
Chapters
00:04
Introduction
01:09
Production of Naive Bovine Monocyte-Derived Dendritic Cells (MoDCs)
06:01
Generation of Antigen-Pulsed MoDCs
07:20
MoDC Lymphocyte Co-Culture
08:20
Flow Cytometric Analysis
09:47
Results: In Vitro Generation of Cattle MoDCs for the Evaluation of Candidate Vaccine Antigens
12:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方法描述了牛单核细胞来源的树突状细胞(MoDC)的产生及其在牛潜在兽用疫苗开发过程中抗原候选物体
外评估中的
应用。
Tags
Bovine
Monocyte-derived Dendritic Cells
Vaccine Immunogenicity
In Vitro Assay
Antigen-presenting Cells
Vaccine Efficacy
Quality Control
Adjuvant Selection
Peripheral Blood Mononuclear Cells
CD14
Flow Cytometry
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