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Immunology and Infection
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在气液界面上生长的原代鼻上皮细胞的感染,以表征人类冠状病毒与宿主的相互作用
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
Please note that all translations are automatically generated.
Click here for the English version.
在气液界面上生长的原代鼻上皮细胞的感染,以表征人类冠状病毒与宿主的相互作用
DOI:
10.3791/64868-v
•
09:02 min
•
September 22, 2023
•
Clayton J. Otter
2
,
Alejandra Fausto
2
,
Li Hui Tan
4
,
Susan R. Weiss
2
,
Noam A. Cohen
4
1
Department of Microbiology
,
University of Pennsylvania
,
2
Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine
,
University of Pennsylvania
,
3
Otorhinolaryngology-Head and Neck Surgery
,
University of Pennsylvania
,
4
Corporal Michael J. Crescenz VA Medical Center
Chapters
00:04
Introduction
00:57
Infection of Nasal Air‐Liquid Interface (ALI) Cultures
02:53
Collection and Quantification of the Shed Virus in Apical Surface Liquid (ASL)
03:49
Transepithelial Electrical Resistance (TEER) Measurement
05:25
Measuring Cytotoxicity by Lactate Dehydrogenase (LDH) Assay
06:18
Results: Characterizing Viral Replication, Host Cell Tropism, and Virus‐Induced Cytotoxicity in Nasal ALI Cultures
08:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
鼻上皮是所有呼吸道病原体遇到的主要屏障部位。在这里,我们概述了使用作为气液界面 (ALI) 培养物生长的原代鼻上皮细胞来表征生理相关系统中人类冠状病毒与宿主相互作用的方法。
Tags
Air-liquid Interface
Nasal Epithelial Cells
Human Coronavirus
Virus Infection
Viral Replication
Host-virus Interactions
Innate Immune Response
Cytotoxicity
Organoid Culture
Airway Epithelium
Asthma
Cystic Fibrosis
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