A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Herbert Wertheim School Optometry and Vision Science,University of California, Berkeley, 2Department of Ophthalmology,Osaka University Graduate School of Medicine, 3Department of Ophthalmology, Tokyo Medical Center,National Hospital Organization, 4Department of Optometry and Vision Science, School of Optometry,University of Alabama at Birmingham
Chapters
- 00:05Introduction
- 00:59Isolating the Ocular Posterior Eye Cup and the RPE/Choroid/Sclera Complex
- 01:57Isolation of the RPE from the Choroid
- 03:47Results: Assessing the Quality of the Isolated RPE Samples
- 04:29Conclusion
Wir beschreiben eine einfache und effiziente Methode zur Isolierung von Zellen der retinalen Pigmentepithelzellen (RPE) aus den Augen junger pigmentierter Meerschweinchen. Dieses Verfahren ermöglicht molekularbiologische Folgeuntersuchungen am isolierten RPE, einschließlich Genexpressionsanalysen.
