A subscription to JoVE is required to view this content. Sign in or start your free trial.
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy,Chengdu University of Traditional Chinese Medicine, 2Chengdu Techman Instrument Co., Ltd., 3Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, 4Research Institute of Integrated TCM & Western Medicine,Chengdu University of Traditional Chinese Medicine, 5School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine
Chapters
- 00:04Introduction
- 01:04Cell Culture
- 01:41Fabrication of Micropipettes
- 02:16Instrument Setup
- 02:37IKv Parameter Setting
- 03:16Whole‐Cell Patch‐Clamp Recording of the IKv in Voltage‐Clamp Mode
- 06:43Results: IKv in H9c2 Cells
- 07:37Conclusion
Tags
Voltage-dependent Potassium CurrentWhole-cell Patch-clamp TechniqueH9C2 CardiomyocytesIon Channel ResearchPharmacological MechanismsCardiotoxicityMicropipette PullerBorosilicate Glass CapillaryDigital Analog ConverterSignal AmplifierMicro ManipulatorData Acquisition SoftwareLeak SubtractionMembrane Test
