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1Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology,Shanghai Jiao Tong University, 2Joint Center for Single Cell Biology, School of Agriculture and Biology,Shanghai Jiao Tong University, 3Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education),Shanghai Jiao Tong University
Chapters
- 00:05Introduction
- 00:36Plant Culture
- 01:20Tissue Preparation and Pretreatment
- 02:37Setting up the Protocol with the Microplate Reader, Establishing the Elicitation System, and Measuring Real-Time ROS Production
- 03:42Results: ROS Induction, the Total Amount of ROS Generated, and ROS Production in the Exposed Cells of the Cut Edge
- 05:07Conclusion
Aqui, descrevemos um método para a detecção em tempo real da produção de espécies reativas de oxigênio (EROs) apoplásicas em tecidos de arroz em resposta imune desencadeada por padrão molecular associado a patógenos. Este método é simples, padronizado e gera resultados altamente reprodutíveis em condições controladas.
