Journal
/
Immunology and Infection
/
将IL-33刺激的巨噬细胞过继转移到博来霉素诱导的小鼠模型中，以研究其对体内特发性肺纤维化的影响
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo
Please note that all translations are automatically generated. Click here for the English version.

将IL-33刺激的巨噬细胞过继转移到博来霉素诱导的小鼠模型中，以研究其对体内特发性肺纤维化的影响

DOI:
10.3791/64742-v

•

06:29 min

•

May 05, 2023

•

, ,
1Department of Basic Medicine, Wuxi School of Medicine,Jiangnan University

Chapters

  • 00:04Introduction
  • 00:39Preparation of Recipient Mouse
  • 01:13Isolation and Culture of Interstitial Macrophages (IMs)
  • 03:13Adoptive Transfer of IMs into the Lung Alveoli
  • 05:12Results: Effect of IL‐33‐Stimulated Macrophages on Idiopathic Pulmonary Fibrosis
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了肺间质巨噬细胞（IMs）的分离及其在小鼠模型中IL-33刺激肺泡后的过继转移，这可以促进特发性肺纤维化（IPF）的 体内 研究。

Tags

Adoptive TransferIL-33MacrophagesBleomycinIdiopathic Pulmonary FibrosisIn VivoClodronate LiposomesAlveolar MacrophagesDMEMCollagenase ACell StrainerCell Lysis BufferHemocytometerInterstitial Macrophages

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

实验转移和CTL过继转移免疫小鼠模型

在实验性自身免疫性脑脊髓炎（EAE）抗性品系小鼠继转移和抗原挑战克服反应迟钝

功能研究和过继转移实验小鼠骨髓嗜中性粒细胞的分离，纯化及贴标

使用<em>福氏痢疾杆菌</em>研究自噬，细胞骨架相互作用

诱导小鼠肠道炎症由效应CD4的过继转移<sup> +</sup> CD45RB<sup>高</sup> T细胞免疫缺陷小鼠

淋巴细胞微粒和检测其促凋亡作用的气道上皮细胞产生

重组流感疫苗的嵌合体小鼠模型鼻腔给药研究黏膜免疫

的气管内管理<em>流感嗜血杆菌</em>在小鼠模型来研究气道炎症

嗜肺军团菌外膜囊泡：隔离及对巨噬细胞促炎潜力分析

IL-8瞬时转化的小鼠模型<em>体内</em>长期监测炎症反应

Read Article