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Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity
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Cancer Research
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JoVE Journal Cancer Research
Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

DOI:
10.3791/64713-v

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09:59 min

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March 31, 2023

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, , ,
1Department of Chemistry,University of Illinois Urbana-Champaign, 2Department of Biochemistry,University of Illinois Urbana-Champaign, 3Beckman Institute for Advanced Science and Technology,University of Illinois Urbana-Champaign, 4Cancer Center of Illinois,University of Illinois Urbana-Champaign

Chapters

  • 00:04Introduction
  • 00:38Fluorescence Microscopy Imaging of Cells with High ALDH1A1 Activity
  • 03:03Application of Flow Cytometry to Identify Cells with High ALDH1A1 Activity
  • 07:20Results: Total ALDH1A1 Activity and Identifying the Population of ALDH1A1+ Cells
  • 09:33Conclusion

Summary

Automatic Translation

Automatic Translation

Methods to measure ALDH1A1 activity in live cells are critical in cancer research due to its status as a biomarker of stemness. In this study, we employed an isoform-selective fluorogenic probe to determine the relative levels of ALDH1A1 activity in a panel of five ovarian cancer cell lines.

Tags

ALDH1A1Stem Cell BiomarkerAlDeSenseFlow CytometryLive Cell ImagingFluorogenic ProbeConfocal MicroscopyCell CultureCell CountingCell DetachmentTrypsinImage Analysis

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