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Journal
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Immunology and Infection
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功能性破骨细胞与人外周血CD14
+
单核细胞的鉴别
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14
+
Monocytes
Please note that all translations are automatically generated.
Click here for the English version.
功能性破骨细胞与人外周血CD14
+
单核细胞的鉴别
DOI:
10.3791/64698-v
•
11:52 min
•
January 27, 2023
•
Patricia Riedlova*
1
,
Shatakshi Sood*
1
,
Carl S. Goodyear
,
Cecilia Ansalone
1
School of Infection & Immunity
,
University of Glasgow
Chapters
00:04
Introduction
00:56
Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Whole Blood
02:34
Enrichment of CD14
+
Monocytes from PBMCs
04:20
Osteoclast Differentiation In Vitro
05:46
TRAP Staining for Osteoclasts
07:10
Actin Ring Fluorescent Staining
09:40
Results: Differentiation of Osteoclasts and Assessing the Function of Mature Osteoclasts
11:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
破骨细胞是体内关键的骨吸收细胞。该协议描述了一种从人外周血单核细胞体
外
分化的破骨细胞的可靠方法。该方法可作为进一步了解体内平衡和疾病中破骨细胞生物学的重要工具。
Tags
Osteoclast Differentiation
PBMC Isolation
CD14+ Monocyte Enrichment
In Vitro Osteoclast Generation
Cytokine Stimulation
Bone Resorption
Arthritis
Therapeutic Screening
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