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Journal
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Immunology and Infection
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マイクロバブルを用いたヒト末梢血単核細胞サンプルからの浮遊選鉱ベースのT細胞の単離、活性化、および増殖(英語)
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles
Please note that all translations are automatically generated.
Click here for the English version.
マイクロバブルを用いたヒト末梢血単核細胞サンプルからの浮遊選鉱ベースのT細胞の単離、活性化、および増殖(英語)
DOI:
10.3791/64573-v
•
06:37 min
•
December 23, 2022
•
Tiffany Snow
,
Jon Roussey
,
Casey Wegner
,
Brandon McNaughton
1
Research and Development
,
Akadeum Life Sciences
,
2
Akadeum Life Sciences
Chapters
00:04
Introduction
00:29
Isolation of T Cells with Microbubbles Using Positive Selection
01:51
Co-Stimulation (Activation) of the Positively Selected T Cells
02:52
Expansion of the Co-Stimulated Cells in Cell Culture Medium
03:28
Expansion of the T Cells (With or Without Prior Transduction)
04:02
Harvesting the T Cells and Flow Cytometry
05:16
Results: Assessment of Viable and Successfully Transduced Cells
05:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
この研究の目的は、初代ヒトT細胞を単離、活性化、および増殖させるための浮遊選鉱ベースの分離の実現可能性を実証することです。
Tags
T Cell Isolation
T Cell Activation
T Cell Expansion
Microbubbles
Buoyancy-based Selection
PBMC
CD3 Antibody
CD28 Antibody
Cell Therapy
Cell Manufacturing
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