A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Pharmacology, College of Medicine,The University of Arizona, Tucson, 2Department of Anesthesiology, College of Medicine,The University of Arizona, Tucson, 3Neuroscience Graduate Interdisciplinary Program, College of Medicine,The University of Arizona, Tucson, 4Comprehensive Pain and Addiction Center,The University of Arizona, Tucson, 5Department of Anesthesiology and Pain Management,University of Texas Southwestern Medical Center
Chapters
- 00:05Introduction
- 00:57First Step: Measure the Baseline of Mechanical Sensitivity Using the Von Frey Test
- 01:53Second Step: Measure the Baseline of Thermal Sensitivity Using the Hargreaves Test
- 02:41Measuring the Baseline of Thermal Sensitivity Using the Hot Plate Test
- 03:47Preoperative Preparation
- 04:30Surgical Procedure and Postsurgical Behavioral Tests
- 06:38Results: Dose‐Dependent Reversal of pSNL‐Induced Mechanical Hypersensitivity by Intraperitoneal Injection of Morphine or Ibuprofen
- 07:36Conclusion
La ligadura parcial del nervio ciático induce dolor neuropático crónico de larga duración, caracterizado por respuestas exageradas a estímulos térmicos y mecánicos. Este modelo de ratón de dolor neuropático se usa comúnmente para estudiar terapias innovadoras para el manejo del dolor. Este artículo describe en detalle el procedimiento quirúrgico para mejorar la estandarización y la reproducibilidad.
