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从羊膜中提取Lumican及其储存温度的测定
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Medicine
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JoVE Journal
Medicine
Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
Please note that all translations are automatically generated.
Click here for the English version.
从羊膜中提取Lumican及其储存温度的测定
DOI:
10.3791/64460-v
•
04:21 min
•
October 14, 2022
•
Luis Haro-Morlett*
1
,
Fatima Sofia Magaña-Guerrero*
1
,
Beatriz Buentello Volante
,
Yonathan Garfias
2
1
Instituto de Oftalmologia Fundacion Conde de Valenciana IAP
,
2
Biochemistry Department, Medicine Faculty
,
Universidad Nacional Autónoma de Mexico
Chapters
00:05
Introduction
00:37
Preparation of the Amniotic Membrane Extract
02:48
Results: Determination of Storage Temperature for Amniotic Membrane Extract
03:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了从羊膜(AM)中提取lumican及其储存条件作为AM提取物(AME)在-20°C,4°C和室温(RT)下6,12,20和32天以量化其蛋白质和lumican浓度。
Tags
Lumican
Amniotic Membrane
Protein Extraction
Balanced Salt Solution
Dispase II
De-epithelialization
Liquid Nitrogen
Protease Inhibitor
Centrifugation
Storage Temperature
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