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评估从3D多细胞肺肿瘤球体中分离的癌症相关成纤维细胞的线粒体健康
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Cancer Research
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JoVE Journal Cancer Research
Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids
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评估从3D多细胞肺肿瘤球体中分离的癌症相关成纤维细胞的线粒体健康

DOI:
10.3791/64315-v

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10:26 min

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October 21, 2022

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, , ,
1Department of Biomedical Engineering,Indian Institute of Technology Ropar

Chapters

  • 00:04Introduction
  • 01:13Preparation of Cell Suspension
  • 03:24Live-Dead Analysis of Tumor Spheroids
  • 04:05Disintegration and Cell Suspension of Tumor Spheroids
  • 05:02Cancer-Associated Fibroblast (CAF) Isolation Through Microbeads
  • 06:19Flow Cytometry-Based Analysis of ACTA2 Expression in Isolated CAFs
  • 07:28Results: Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

用肺腺癌细胞、成纤维细胞和单核细胞制备多细胞3D肿瘤球体，然后从这些球体中分离癌症相关成纤维细胞（CAF）。将分离的CAF与正常成纤维细胞进行比较，通过研究线粒体跨膜电位，活性氧和酶活性来评估线粒体健康状况。

Tags

Cancer-associated FibroblastsCAF3D Multicellular Lung Tumor SpheroidsMitochondrial HealthTumor MicroenvironmentIn Vitro ModelCell IsolationCell CultureA549 CellsMRC-5 CellsTHP-1 CellsTumor Spheroid Formation

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