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Cancer Research
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一种基于流式细胞术的细胞表面蛋白结合测定,用于评估抗癌适配体的选择性和特异性
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
Please note that all translations are automatically generated.
Click here for the English version.
一种基于流式细胞术的细胞表面蛋白结合测定,用于评估抗癌适配体的选择性和特异性
DOI:
10.3791/64304-v
•
10:46 min
•
September 13, 2022
•
Maryam Nakhjavani
2
,
Breanna Giles
2
,
Mia Strom
2
,
Chris Vi
2
,
Sahara Attenborough
2
,
Sarah Shigdar
2
1
School of Medicine
,
Deakin University
,
2
Institute for Mental and Physical Health and Clinical Translation, School of Medicine
,
Deakin University
Chapters
00:05
Introduction
01:02
Binding Assay
04:10
Flow Cytometry and Data Analysis
08:58
Results: Flow Cytometric-Based Assay to Study the Binding of an Aptamer and Cancer Cells
09:55
Conclusion
Summary
Automatic Translation
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Automatic Translation
抗癌适配体开发的必要步骤是测试其与靶标的结合。我们展示了一种基于流式细胞术的测定来研究这种结合,强调了包括阴性对照适配体和对该特定蛋白质呈阳性或阴性的癌细胞的重要性。
Tags
Flow Cytometry
Cell Surface Protein
Aptamer
Anticancer
Selectivity
Specificity
Immunophenotyping
Multi-parametric
Cell Culture
Trypsinization
Cell Counting
Cell Stabilization
Blocking Buffer
Aptamer Folding
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