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Journal
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Immunology and Infection
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从P3HR1细胞系分离和定量EB病毒
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Please note that all translations are automatically generated.
Click here for the English version.
从P3HR1细胞系分离和定量EB病毒
DOI:
10.3791/64279-v
•
09:14 min
•
September 28, 2022
•
Elio R. Bitar
,
Marcel S. Shams Eddin
,
Elias A. Rahal
2
1
Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine
,
American University of Beirut
,
2
Center for Infectious Diseases Research (CIDR), Faculty of Medicine
,
American University of Beirut
Chapters
00:05
Introduction
00:36
Preparing the Plate for Culture
01:49
Induction and Isolation of Epstein-Barr Virus Particles
02:45
DNA Extraction from Viral Particles
04:45
Checking DNA Concentration and Purity
05:34
Quantification by Real-Time Polymerase Chain Reaction
07:54
Results: Cell Counting and Induction of P3HR-1 Cells with PMA and DMSO
08:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议允许在用佛波醇12-肉豆蔻酸酯13-乙酸酯诱导病毒裂解循环时从人P3HR1细胞系中分离Epstein-Barr病毒颗粒。随后从病毒制剂中提取DNA,并进行实时PCR以量化病毒颗粒浓度。
Tags
Epstein-Barr Virus
P3HR1 Cell Line
Viral Stock
Virus Isolation
Virus Quantification
PMA
DMSO
Cell Culture
Centrifugation
Phenol-chloroform Extraction
Ethanol Precipitation
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