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合成mRNA转染后肿瘤细胞迁移和侵袭的实时定量测量
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Cancer Research
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JoVE Journal Cancer Research
Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection
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合成mRNA转染后肿瘤细胞迁移和侵袭的实时定量测量

DOI:
10.3791/64274-v

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11:00 min

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June 23, 2023

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,
1Children’s Mercy Research Institute,Children’s Mercy Kansas City, 2Department of Pediatrics,University of Missouri-Kansas City School of Medicine

Chapters

  • 00:04Introduction
  • 00:56Synthesis of mRNA
  • 03:57Extracellular Matrix (ECM) Gel Coating of the Cell Invasion and Migration (CIM) Plates
  • 04:38Electroporation of the Tumor Cells
  • 05:40Setting Up the Real‐Time Cell Analyzer, the Program, and the CIM Plates
  • 07:29Real‐Time Cell Analysis and Data Export
  • 08:23Results: Tumor Cell Migration and Invasion
  • 10:17Conclusion

Summary

Automatic Translation

Automatic Translation

许多上调的基因刺激肿瘤细胞迁移和侵袭，导致预后不良。确定哪些基因调节肿瘤细胞迁移和侵袭至关重要。该协议提出了一种实时研究基因表达增加对肿瘤细胞迁移和侵袭的影响的方法。

Tags

Tumor Cell MigrationTumor Cell InvasionReal-time Quantitative MeasurementSynthetic MRNA TransfectionImpedance-based Real-time Cell AnalysisGene ExpressionMetastasisCancer TherapyRNA SynthesisLithium Chloride PrecipitationCappingPoly-A Tailing

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