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Cancer Research
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小鼠胸腺细胞在δ样4表达基质细胞上的共培养和转导以研究T细胞白血病的癌基因
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia
Please note that all translations are automatically generated.
Click here for the English version.
小鼠胸腺细胞在δ样4表达基质细胞上的共培养和转导以研究T细胞白血病的癌基因
DOI:
10.3791/64271-v
•
09:43 min
•
June 09, 2023
•
Gisele O. L. Rodrigues
,
WenQing Li
,
Sarah D. Cramer
2,3
,
Hila Y. Winer
,
Tu Chun Hsu
2,3
,
Timothy Gower
,
Julie A. Hixon
,
Scott K. Durum
1
Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute
,
National Institutes of Health
,
2
Comparative Biomedical Scientist Training Program
,
NIH
,
3
Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory
,
Michigan State University
,
4
NCI-Frederick Laboratory Animal Sciences Program
Chapters
00:04
Introduction
00:47
Seeding and Transfecting the Retrovirus-Producer Cells
02:31
Thymocyte Preparation and Depletion of CD4
+
and CD8
+
Cells
04:53
Harvesting Retrovirus and Transducing the Thymocytes
07:13
Results: Thymocyte Depletion and Thymocyte Retroviral Transduction Efficiency
08:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了从小鼠胸腺中分离双阴性胸腺细胞,然后在δ样4表达骨髓基质细胞系共培养系统(OP9-DL4)上进行逆转录病毒转导和共培养以进行进一步的功能分析。
Tags
Co-culture
Transduction
Murine Thymocytes
Delta-Like 4
Stromal Cells
T-cell Leukemia
Retroviral Vectors
OP9 DL4
In Vitro System
T-cell Development
Cancer
Thymocyte Suspension
Thymus
PBS
Glass Slides
Cell Filtration
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