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Journal
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Neuroscience
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体内
使用大颅窗的小鼠宽场和双光子钙成像
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
In Vivo
Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
Please note that all translations are automatically generated.
Click here for the English version.
体内
使用大颅窗的小鼠宽场和双光子钙成像
DOI:
10.3791/64224-v
•
06:45 min
•
August 04, 2022
•
Satoshi Manita
,
Eiji Shigetomi
3
,
Haruhiko Bito
,
Schuichi Koizumi
3
,
Kazuo Kitamura
1
Department of Neurophysiology, Faculty of Medicine
,
University of Yamanashi
,
2
Department of Neuropharmacology, Faculty of Medicine
,
University of Yamanashi
,
3
Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine
,
University of Yamanashi
,
4
Department of Neurochemistry, Graduate School of Medicine
,
The University of Tokyo
Chapters
00:04
Introduction
00:51
Cranial Window Construction
03:32
Making AAV Film on Plastic Wrap Using Fibroin Solution
04:17
Results: Evaluation of Cranial Window Made Using the PVDC Wrap Methods
05:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了使用食品包装,透明硅胶和盖玻片制作大型(6 x 3 mm
2
)颅窗。该颅窗允许在同一只小鼠中进行
体内
宽场和双光子钙成像实验。
Tags
Cranial Window
Wide-field Calcium Imaging
Two-photon Calcium Imaging
Mouse
Neural Activity
Glial Activity
Behavior
Macroscopic
Microscopic
PVDC Wrap
ACSF
Biological Adhesive
Silicone Elastomer
Cover Glass
Fibroin
AAV
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