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从原代细胞和培养细胞制备细胞质和核长RNA
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Cancer Research
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JoVE Journal
Cancer Research
Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
Please note that all translations are automatically generated.
Click here for the English version.
从原代细胞和培养细胞制备细胞质和核长RNA
DOI:
10.3791/64199-v
•
06:29 min
•
April 07, 2023
•
Jacob Jahn
,
Sana Chaudhry
,
Maurizio Affer
,
Alejandro Pardo
,
Gabriel Pardo
,
Justin Taylor
1
Sylvester Comprehensive Cancer Center
,
University of Miami Miller School of Medicine
Chapters
00:04
Introduction
01:11
Separation of Nuclear and Cytoplasmic Fractions
01:56
Validation of Nuclear-Cytoplasmic Separation
04:35
Results: Confirmation of RNA Purity of Cytoplasmic and Nuclear Fraction
05:48
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议提供了一种高效灵活的方法,使用培养的细胞从核和细胞质级分中分离RNA,然后使用qPCR进行验证。这有效地替代了其他RNA制备试剂盒。
Tags
Cytoplasmic RNA
Nuclear RNA
Cell Fractionation
Lysis Buffer
QPCR
MALAT1
TUG1
Cell Culture
Intracellular Interactions
Nuclear-cytoplasmic Exchange
Cancer Development
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