JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
东方果蝇
背侧芽孢
杆菌CRISPR/Cas9诱变实验方案
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly
Bactrocera dorsalis
Please note that all translations are automatically generated.
Click here for the English version.
东方果蝇
背侧芽孢
杆菌CRISPR/Cas9诱变实验方案
DOI:
10.3791/64195-v
•
08:19 min
•
September 28, 2022
•
Jinxi Yuan
,
Jie Zhang
,
Yan Zhang
,
WuYun QiQiGe
,
Wei Liu
,
Shanchun Yan
,
Guirong Wang
1
Key Laboratory of Sustainable Forest Ecosystem Management – Ministry of Education
,
Northeast Forestry University
,
2
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen
,
Chinese Academy of Agricultural Sciences
Chapters
00:05
Introduction
00:33
Target Design and In Vitro Synthesis of sgRNA
01:18
Embryo Collection and Preparation
02:39
Microinjection of Embryos
04:24
Post-Injection Insect Rearing
05:07
Mutant Screening
06:41
Results: Mutant Screening in
B. dorsalis
07:39
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文介绍了东方果蝇背
蝇
CRISPR/Cas9诱变的分步方案。该标准化协议提供的详细步骤将作为产生突变果蝇的有用指南,用于背
双歧杆菌
的功能基因研究。
Tags
CRISPR/Cas9
Mutagenesis
Oriental Fruit Fly
Bactrocera Dorsalis
Functional Gene Research
Pest Management
GRNA
Embryo Collection
Microinjection
Cas9 Protein
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
学习在果蝇的侵略(果蝇)
导演蛋白质的演化突变和功能选择协议
E。大肠杆菌</em
果实角质层分离和生物物理研究
基因缺失在通过CRISPR / Cas9哺乳动物细胞系生成
沉默的火花:CRISPR/Cas9基因组编辑在弱电鱼
准备和注射
Culex
蚊子的胚胎,使用CRISPR/Cas9产生空突变
使用CRISPR/Cas9的人类B细胞基因组工程
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
造血干细胞和祖细胞的CRISPR/Cas9基因编辑用于基因治疗应用
CRISPR-Cas9介导的斑马鱼心脏中的精确敲入编辑
Read Article