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用于下游无循环DNA应用的标准化液体活检前分析方案
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
DOI:

05:26 min

September 16, 2022

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Chapters

  • 00:05Introduction
  • 01:30Serum Preparation for Liquid Biopsy Studies
  • 02:25Plasma Preparation for Liquid Biopsy Studies
  • 04:08Results: Electrophoresis-Based Analysis of cfDNA Isolated from Plasma
  • 04:28Conclusion

Summary

Automatic Translation

液体活检彻底改变了我们的肿瘤学转化研究方法,样本收集、质量和储存是其成功临床应用的关键步骤。在这里,我们描述了一种标准化且经过验证的下游无循环DNA应用的方案,该协议可应用于大多数转化研究实验室。

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