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Cancer Research
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用于下游无循环DNA应用的标准化液体活检前分析方案
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Cancer Research
A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
Please note that all translations are automatically generated.
Click here for the English version.
用于下游无循环DNA应用的标准化液体活检前分析方案
DOI:
10.3791/64123-v
•
05:26 min
•
September 16, 2022
•
Julie Earl
,
Silvia Calabuig-Fariñas
,
María Eugenia Sarasquete
,
Laura Muinelo Romay
,
Sara Lopez-Tarruella
,
Beatriz Bellosillo Paricio
,
Marta Rodríguez
,
Karmele Valencia Leoz
,
Marta Dueñas Porto
,
Noelia Tarazona
,
Javier Hernandez Losa
,
Rodrigo Almeida Toledo
1
The Liquid Biopsy and Biomarker Working Module
,
the Biomedical Research Network in Cancer (CIBERONC)
Chapters
00:05
Introduction
01:30
Serum Preparation for Liquid Biopsy Studies
02:25
Plasma Preparation for Liquid Biopsy Studies
04:08
Results: Electrophoresis-Based Analysis of cfDNA Isolated from Plasma
04:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
液体活检彻底改变了我们的肿瘤学转化研究方法,样本收集、质量和储存是其成功临床应用的关键步骤。在这里,我们描述了一种标准化且经过验证的下游无循环DNA应用的方案,该协议可应用于大多数转化研究实验室。
Tags
Liquid Biopsy
Circulating Free DNA
Preanalytical Protocol
Blood Sample Processing
Plasma Extraction
Serum Extraction
Sample Centrifugation
Sample Storage
Hemolysis Detection
Standardized Procedure
Translational Research
Clinical Applications
Oncology Research
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