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用于肿瘤球体半高通量分析的快速光学清除
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids
Please note that all translations are automatically generated.
Click here for the English version.
用于肿瘤球体半高通量分析的快速光学清除
DOI:
10.3791/64103-v
•
08:12 min
•
August 23, 2022
•
Gency Gunasingh
,
Alexander P. Browning
,
Nikolas K. Haass
1
The University of Queensland Diamantina Institute
,
The University of Queensland
,
2
School of Mathematical Sciences
,
Queensland University of Technology
Chapters
00:04
Introduciton
00:43
Preparation of 2% Agarose‐PBS Gel and Clearing Solution
01:39
Spheroid Preparation and Staining
04:04
Spheroid Mounting and Imaging
05:46
Results: Semi-High‐Throughput Imaging of 3D Tumor Spheroids
07:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肿瘤球体越来越多地用于评估肿瘤细胞 - 微环境相互作用和治疗反应。本方案描述了一种使用快速光学清除对3D肿瘤球体进行半高通量成像的稳健但简单的方法。
Tags
Tumor Spheroids
3D Tumor Modeling
Optical Clearing
Rapid Clearing
Semi-high-throughput Analysis
Agarose Overlay Method
Paraformaldehyde Fixation
Antibody Staining
Triton X-100 Permeabilization
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