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Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology
JoVE Journal
Biology
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JoVE Journal Biology
Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology
DOI:

06:54 min

July 05, 2022

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Chapters

  • 00:04Introduction
  • 00:36Cell Culture with Magnetic Microbeads
  • 01:29Force Application and Live-Cell Imaging
  • 04:34Results: Application of Mechanical Force on Cell Nucleus and Simultaneous Live-Cell Fluorescent Imaging
  • 06:28Conclusion

Summary

Automatic Translation

This study presents a new protocol to directly apply mechanical force on the cell nucleus through magnetic microbeads delivered into the cytoplasm and to conduct simultaneous live-cell fluorescent imaging.

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