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Journal
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Immunology and Infection
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用于测试新兴抗生素的新型高通量
离体
绵羊皮肤伤口模型
JoVE Journal
Immunology and Infection
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Immunology and Infection
A Novel High-Throughput
Ex Vivo
Ovine Skin Wound Model for Testing Emerging Antibiotics
Please note that all translations are automatically generated.
Click here for the English version.
用于测试新兴抗生素的新型高通量
离体
绵羊皮肤伤口模型
DOI:
10.3791/64041-v
•
08:30 min
•
September 16, 2022
•
Hannah C. Regan
2
,
Annette F. Taylor
2
,
Esther Karunakaran
2
1
Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB)
,
University of Sheffield
,
2
Department of Chemical and Biological Engineering
,
University of Sheffield
Chapters
00:05
Introduction
00:53
Sterilization and Preparation of the Head
03:06
Maintenance of Skin Samples
04:18
Infection of Skin Samples
05:27
Determination of the Bacterial Load
06:32
Results: An Ex Vivo Ovine Wounded Skin Model Infected with
Staphylococcus aureus
07:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种逐步建立感染
金黄色葡萄球菌
的离
体
绵羊受伤皮肤模型的方法。与传统微生物学技术相比,这种高通量模型更好地模拟
体内
感染,并为研究人员提供了一个生理相关的平台来测试新兴抗菌剂的功效。
Tags
Ovine Skin Wound Model
High-throughput Ex Vivo
Antimicrobial Efficacy
Topical Antimicrobials
Wound Infections
Contamination
Aseptic Technique
Skin Sample
Chlorine Dioxide Solution
Sterile PBS
Air-liquid Interface
Article
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