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Isolamento e Análise de Vesículas Extracelulares Rastreáveis e Funcionalizadas do Plasma e Tecidos Sólidos
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
DOI:

09:57 min

October 17, 2022

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Chapters

  • 00:05Introduction
  • 01:20Preparation of the Plasma and the Maxillary Bone Samples and Extraction of Extracellular Vesicles
  • 02:50Origin Analysis of Extracellular Vesicles
  • 05:17Protein Content Analysis of Extracellular Vesicles
  • 08:06Results: Profiling of Surface Antigens and Protein Cargos of Extracellular Vesicles Isolated from In Vivo Samples
  • 09:13Conclusion

Summary

Automatic Translation

O presente protocolo descreve um método para extração de vesículas extracelulares do sangue periférico e tecidos sólidos com posterior perfilamento de antígenos de superfície e cargas proteicas.

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